Thoughts

[fuggersbergerdavid]

• how exact is ngs sequencing? is maxlenght of templates to strict?
• using single reads and multiple reads? are those errors or actual reads that are verifed? dont use them as template but consider read coverage of uncloneable region/prot for single and multiple reads
• cutoff for uncloneable regions 10? (set manual depending on average coverage of stems? High average coverage, higher cutoff?)
• uncloneable equales 0 template coverage; decreased equals 1 or 2 template coverage? setting higher values if higher average coverage?
• more detailed output for decreased prot or leave it this way because it probably gets solved by integrating in pedant